Clsi M22a3: Pdf

CLSI M22-A3 standard, titled "Quality Control for Commercially Prepared Microbiological Culture Media; Approved Standard—Third Edition,"

Standard MacConkey Agar, Sheep Blood Agar (for general growth), and Chocolate Agar.

Always verify if your specific accreditation body (e.g., CAP) requires adherence to the historical M22-A3 criteria or mandates an upgrade to the latest edition. clsi m22a3 pdf

Anaerobic media (e.g., Brucella blood agar) must be tested with anaerobes (e.g., Bacteroides fragilis ) and, as a negative control, an aerobe that should not grow (e.g., Pseudomonas aeruginosa ). Testing frequency—lot, shipment, or day—depends on risk assessment, but daily testing is common in high-complexity labs.

The primary goal of this document is to streamline the QC testing process in clinical laboratories. It achieves this by categorizing media based on historical failure rates. This allows laboratories to reduce redundant testing for highly stable, low-failure media while maintaining rigorous testing for more complex, failure-prone media. Key Objectives and Scope This allows laboratories to reduce redundant testing for

For each organism, the standard specifies the ATCC or NCTC strain number, incubation conditions, and minimal expected performance.

Test for using negative control organisms that should be inhibited. Non-Exempt media includes complex

Non-Exempt media includes complex, selective, or highly sensitive formulations that are prone to deterioration during shipping or storage. Laboratories must perform full, independent quality control testing on every new lot of non-exempt media before using it for patient diagnostics. Examples of non-exempt media include: Campylobacter media (e.g., Campy-CVA)

: It includes new recommendations for the quality control of media used to grow "fastidious" (difficult to grow) organisms. Clinical & Laboratory Standards Institute | CLSI Why This Matters for Laboratories

: The acceptable extrapolated failure rate for media to qualify as exempt was increased from 0.3% to 0.5% Defined Responsibilities

Selective media for pathogenic Neisseria (e.g., Modified Thayer-Martin) Certain specialized broths and transport media Steps for Implementing CLSI M22-A3 in Your Lab Step 1: Visual Inspection and Log-In